Agent skill · research science · k-dense-ai
glycoengineering
Analyze and engineer protein glycosylation. Scan sequences for N-glycosylation sequons (N-X-S/T), predict O-glycosylation hotspots, and access curated glycoengineering tools (NetOGlyc, GlycoShield, GlycoWorkbench). For glycoprotein engineering, therapeutic antibody optimization, and vaccine design.
Why this skill is useful
Provides domain-specific knowledge and Python scripts for analyzing N-glycosylation sites that the AI wouldn't reliably generate on its own.
What it needs
Requires python installed locally. About 6k tokens when loaded. Last updated 2026-08-03. 32,849 stars on the source repository.
What this skill does
Glycoengineering Overview Glycosylation is the most common and complex post-translational modification (PTM) of proteins, affecting over 50% of all human proteins. Glycans regulate protein folding, stability, immune recognition, receptor interactions, and pharmacokinetics of therapeutic proteins. Glycoengineering involves rational modification of glycosylation patterns for improved therapeutic efficacy, stability, or immune evasion. Two major glycosylation types: N-glycosylation: Attached to asparagine (N) in the sequon N-X-[S/T] where X ≠ Proline; occurs in the ER/Golgi O-glycosylation: Attached to serine (S) or threonine (T); no strict consensus motif; primarily GalNAc initiation When to Use This Skill Use this skill when: Antibody engineering: Optimize Fc glycosylation for enhanced ADCC, CDC, or reduced immunogenicity Therapeutic protein design: Identify glycosylation sites that affect half-life, stability, or immunogenicity Vaccine antigen design: Engineer glycan shields to focus immune responses on conserved epitopes Biosimilar characterization: Compare glycan patterns between reference and biosimilar Drug target analysis: Does glycosylation affect target engagement for a receptor? Protein stability: N-glycans often stabilize proteins; identify sites for stabilizing mutations N-Glycosylation Sequon Analysis Scanning for N-Glycosylation Sites N-glycosylation occurs at the sequon N-X-[S/T] where X ≠ Proline. Mutating N-Glycosylation Sites O-Glycosylation Analysis Heuristic O-Glycosylation Hotspot Prediction External Glycoengineering Tools 1. NetOGlyc 4.0 (O-glycosylation prediction) Web service for high-accuracy O-GalNAc site prediction: URL: https://services.healthtech.dtu.dk/services/NetOGlyc-4.0/ Input: FASTA protein sequence Output: Per-residue O-glycosylation probability scores Method: Neural network trained on experimentally verified O-GalNAc sites 2. …
How to use it
Reference it in AdaL, Claude Code, Cursor or any coding agent — nothing to install:
@skills k-dense-ai/glycoengineering